Figure 1.

Identification of antigen Tp2. (A) Lysis of autologous schizont infected cells by four T. parva-specific CTL lines capable of (B) specifically reacting to iSF transfected with 12 selected genes as assessed by IFN-γ ELISpot and (C) lysis of cDNA Tp01_56 transfected-iSF. (A) Closed circles: BW002; open circles: BW013; closed squares: BW014; and open squares: D409. (B) IFN-γ responses to selected gene transfected iSF are presented as mean numbers of spot forming cells (SFC)/well. Black: BW002; white: BW013; grey: BW014; and hatched: D409. (C) Mean lysis of cDNA Tp01_56 transfected-iSF by CTL from the four cattle. Closed circles: cDNA Tp01_56 transfected autologous iSF; open circles: Irrelevant gene transfected autologous iSF; closed squares: cDNA Tp01_56 transfected autologous iSF pre-incubated with an anti-MHC class I mAb; and open squares: cDNA Tp01_56 transfected allogeneic iSF.