Figure 4.

Ex vivo Tp2 specific CD8+ T cell responses following challenge of immune cattle. Tp2 specific CD8⁺ T cell responses following challenge of T. parva immune cattle. Responses of three cattle, (A) BW014, (B) BW013 & (C) BW002, were measured longitudinally by IFN-γ ELISpot and responses are presented as mean numbers of spot-forming cells (SFC)/1 × 10⁶ CD8⁺ T cells. Closed circles: Tp2 antigenic peptide and open circles: control peptide. (D) Cytotoxic activity of stimulated BW002 PBMC was assessed 14 days post-challenge. Closed circles: Autologous schizont-infected cells; closed squares: Tp2 antigenic peptide pulsed iSF; open squares: Control peptide pulsed iSF; and open circles: Allogeneic schizont infected cells.