Table 1

HLA-Cw4 dataset used in this study.
No.	Category	Source	Peptide	IC₅₀(nM)	Ref.

1	Training Set	Cw3 consensus	FAMPNFQTL	651	24
2	Training Set	Cw6 consensus	IPFPIVRYL	>30000	24
3	Training Set	Cw7 consensus	KYPDFVDAL	2.4	24
4	Training Set	Cw4 consensus	QYDDAVYKL	18	24
5	Training Set	Histone H3.3	RYRPGTVAL	>30000	24
6	Training Set	Unknown Cw6 natural ligand	YQFTGIKKY	>30000	24
7	Test Set	Transcripton factor SUPT4H 56–64	SFDGIIAMM	Binder	10
8	Test Set	Transducin-like 3 120–128	AFDPTSTLL	Binder	10
9	Test Set	UBE3B variant 1 742–750	VFDPALNLF	Binder	10
10	Test Set	XP_173235 3–11	LFDITGQDF	Binder	10
11	Test Set	8 59–67	VYDTNPAKF	Binder	10
12	Test Set	Acyl-CoA synthetase 4 82–90	LFDHAVSKF	Binder	10
13	Test Set	Adenosylhomocyteinehydrolase 566–574	SFDAHLTEL	Binder	10
14	Test Set	ART-1 Adenocarcinoma antigen 21–29	SFDLLPREF	Binder	10
15	Test Set	ATP-binding cassette, sub-family F, member 3 500–508	YYDPKHVIF	Binder	10
16	Test Set	Block of proliferation 1 639–647	SYDSKLVWF	Binder	10
17	Test Set	BM-015 144–152	HFDPEVVQI	Binder	10
18	Test Set	CDC45 541–549	HFDLSVIEL	Binder	10
19	Test Set	Cholesterol acyltransferase 72–80	HFDDFVTNL	Binder	10
20	Test Set	Chromosome 20 open reading frame 40 318–326	FFDNISSEL	Binder	10
21	Test Set	Elongation factor 2 265–273	YFDPANGKF	Binder	10
22	Test Set	Epithelial cell transforming oncogene 21–29	IFDSKVTEI	Binder	10
23	Test Set	Ethanolamine kinase EKI1 132–141	HWDPQEVTL	Binder	10
24	Test Set	Eukaryotic translation initiation factor 3, Su 6 interacting protein 478–486	FLDLTEGEF	Binder	10
25	Test Set	Fatty acid synthetase 544–552	TFDDIVHSF	Binder	10
26	Test Set	FK506 binding protein 9 303–311	VFDIHVIDF	Binder	10
27	Test Set	Glutamine:fructose-6-phosphate amidotransferase (GFAT) 345–353	NFDDYTVNL	Binder	10
28	Test Set	Tousled-like kinase 456–464	AFDLTEQRY	Binder	10
29	Test Set	Transcription factor IIE 144–152	LFDPMTGTF	Binder	10
30	Test Set	HSP 70 kDa 1A 179–205	IFDLGGGTF	Binder	10
31	Test Set	HSPC198 19–27	SYDLFVNSF	Binder	10
32	Test Set	HSP J2 156–164	SFDTGFTSF	Binder	10
33	Test Set	Hypothetical protein FLJ00365 80–88	YFDAIPVTM	Binder	10
34	Test Set	Hypothetical protein FLJ11220 373–381	YLPDFLDYF	Binder	10
35	Test Set	Hypothetical protein FLJ20343 415–423	RFDEAYIYM	Binder	10
36	Test Set	Insuline degrading enzyme IDE 150–158	YFDVSHEHL	Binder	10
37	Test Set	Integrin alpha-V (Vitronectin) 224–232	KYDPNVYSI	Binder	10
38	Test Set	KCIP-1 186–194	AFDEAIAEL	Binder	10
39	Test Set	KIAA0461 721–729	SMDPLPVFL	Binder	10
40	Test Set	KIAA1463 444–452	FYDERIVVV	Binder	10
41	Test Set	KIAA1921 350–358	VYDGKIYTL	Binder	10
42	Test Set	Metalloproteinase 10 331–339	FWPSLPSYL	Binder	10
43	Test Set	Methionine-tRNA synthetase 2 (mitochondrial) 58–66	YYDEKVVKL	Binder	10
44	Test Set	Mucin-5B 168–176	TFDGTSYTF	Binder	10
45	Test Set	Myosin phosphatase target subunit 1 37–45	KFDDGAVFL	Binder	10
46	Test Set	Nuclear autoantigenic sperm protein 599–607	QYDEAVAQF	Binder	10
47	Test Set	Nucleoporin NUP358 261–269	SFDSALQSV	Binder	10
48	Test Set	P621 70–78	VFDKTLAEL	Binder	10
49	Test Set	Phosphate carrier precursor 331–339	IYDSVKVYF	Binder	10
50	Test Set	PRO2242 61–69	YFDPQYFEF	Binder	10
51	Test Set	Protein phosphatase 6 102–110	KWPDRITLL	Binder	10
52	Test Set	Putative prostate tumor suppressor 165–173	TFDLQRIGF	Binder	10
53	Test Set	Rac1 168–176	VFDEAIRAV	Binder	10
54	Test Set	RNA Helicase A 697–705	VFDPVPVGV	Binder	10
55	Test Set	Similiar to KIAA1911 80–88	FWDGKIVLV	Binder	10
56	Test Set	Topoisomerase I 241–249	YYDGKVMKL	Binder	10
57	Test Set	Tensin 3 143–151	FYDDKVSAL	Binder	10
58	Test Set	HIV-1 (BRU) gp120 350–358	SFNCGGEFF	Binder	18
59	Test Set	HIV-1 (BRU) gag p24 307–315	QASQEVKNW	Binder	21
60	Test Set	Synthetic peptide	AYDDAVYKL	Binder	25
61	Test Set	Cw7 consensus	AYADFVYAY	>30000	24
62	Test Set	CKShs2	KYFDEHYEY	>30000	24
63	Test Set	Unknown Cw6 natural ligand	YRHDGGNVL	>30000	24
64	Test Set	Unknown Cw7 natural ligand	NKADVILKY	>30000	24

Tong et al. Immunome Research 2007 3:7 doi:10.1186/1745-7580-3-7