Table 4

GPCRs repressed by LPS in macrophages.



Relative Normalised Expression



Receptor Class
 Accession Number
 Gene Name
 BMM
 TEPM






2 h
 6 h
 24 h
 1 h
 7 h
Repressed by LPS in BMM and TEPM
Class A







NM_009924
 Cnr2
 0.2
 0.1
 0.3
 0.3
 0.1

NM_009911
 Cxcr4
 0.3
 0.1
 7.1
 0.7
 0.1

NM_183031
 Ebi2
 0.2
 0.1
 0.1
 0.4
 0.2

NM_004230
 Edg5
 0.2
 0.5
 2.0
 0.4
 0.6

NM_022320
 Gpr35
 0.2
 0.4
 1.6
 0.6
 0.1

NM_030258
 Gpr146
 0.1
 0.4
 1.0
 0.4
 0.1

NM_008772
 P2ry1
 0.3
 0.1
 0.2
 0.3
 0.1

NM_175116
 P2ry5
 0.1
 0.1
 0.7
 0.3
 0.1

NM_013641
 Ptger1
 0.7
 0.5
 0.3
 0.7
 0.3

NM_008965
 Ptger4
 3.1
 0.5
 1.0
 1.9
 0.3
Repressed by LPS in BMM
Class A







NM_007420
 Adrb2
 0.3
 0.1
 0.1
 1.9
 0.8

NM_009779
 C3ar1
 0.7
 0.5
 0.3
 0.8
 0.6

NM_007577
 C5r1
 1.1
 0.7
 0.1
 3.0
 1.1

NM_009915
 Ccr2
 0.5
 0.4
 0.2
 0.8
 0.9

NM_009910
 Cxcr3
 0.7
 0.1
 0.1
 1.6
 0.9

NM_009987
 Cx3cr1
 0.5
 0.1
 0.1
 1.0
 0.7

NM_030720
 Gpr84
 8.3
 10.0
 0.2
 7.7
 50.0

NM_027571
 P2ry12
 0.5
 0.1
 0.2
 1.0
 1.0
Class B







NM_018782
 Calcrl
 1.0
 2.6
 0.2
 0.6
 7.1
Repressed by LPS in TEPM
Class A







NM_007719
 Ccr7
 1.0
 5.0
 1.9
 0.4
 2.9

NM_021381
 Prokr1
 0.8
 33.3
 7.1
 0.3
 0.1

NM_198168
 Ppp2r5b
 1.0
 1.3
 1.1
 0.8
 0.5

NM_008964
 Ptger2
 2.4
 1.3
 11.1
 0.3
 0.1
Class B







NM_080437
 Celsr3
 0.8
 1.2
 2.3
 0.2
 0.2

NM_173036
 Gpr97
 0.8
 1.0
 0.8
 0.5
 0.2

NM_016894
 Ramp1
 1.0
 1.0
 1.0
 0.5
 0.2
Class C







NM_022420
 Gprc5b
 1.0
 1.0
 1.3
 0.8
 0.1

Micro-array analysis was used to assess the gene expression profiles of GPCRs upon LPS treatment in primary mouse macrophages (BMM and TEPM). GPCRs regulated by LPS in primary mouse macrophages were defined as those that were 2-fold or greater down-regulated at 2, 6 or 24 h LPS in BMM and/or 1 or 7 h LPS in TEPM (and significantly different from controls, p-value < 0.05, Student's two-tailed t-test). Values shown depict gene expression relative to untreated control (0 h). GPCRs are grouped according to their class type on the basis of sequence similarity as previously described [65-67].

Lattin et al. Immunome Research 2008 4:5   doi:10.1186/1745-7580-4-5