Research
Functional recombinant MHC class II molecules and high-throughput peptide-binding assays
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* Corresponding author: Søren Buus sbuus@sund.ku.dk
Laboratory of Experimental Immunology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
Immunome Research 2009, 5:2 doi:10.1186/1745-7580-5-2
Published: 5 May 2009Additional files
Additional file 1:
Additional material. Further information on the following subjects is available as additional material: • Sequences of MHC II constructs used in this work (Figure 1); • Effect of refolding additives (Table 1); • Development of a high throughput scintillation proximity assay (SPA) peptide binding assay (Figure 2); • Selection of DR specific antibodies (Figure 3); • Truncation of DR α and β chains (Figure 4); • The use of the nucleotide exchange factor (GrpE) as a novel MHC II dimerization motif; and • Summary of LOCI assay conditions (Table 2).
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