Figure 8.

Direct and competitive LOCI-based peptide-MHC-II binding assay. Titrations on a promiscuous malaria derived peptide YILLKKILSSRFNQM DR4: K_D = 133 nM R² = 0.98. DP401: K_D = 0.8 nM R² = 0.99. I-E^d: K_D = 14 nM R² = 0.98. DR2a: inhibition assay, reference peptide 4 nM Biotin-PEG-HA_306–318 IC₅₀ = 0.6 nM R² = 0.98.