Additional file 1.

Additional material. Further information on the following subjects is available as additional material: • Sequences of MHC II constructs used in this work (Figure 1); • Effect of refolding additives (Table 1); • Development of a high throughput scintillation proximity assay (SPA) peptide binding assay (Figure 2); • Selection of DR specific antibodies (Figure 3); • Truncation of DR α and β chains (Figure 4); • The use of the nucleotide exchange factor (GrpE) as a novel MHC II dimerization motif; and • Summary of LOCI assay conditions (Table 2).

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Justesen et al. Immunome Research 2009 5:2   doi:10.1186/1745-7580-5-2