Table 1

Affinity measurements using LOCI assay.

Values represent an average of 2–4 measurements. Affinity measurements higher than 5000 nM were categorized as non-binders (NB). N.D: Not determined. a: The DR1 allele used for these measurements was produced as a construct comprising amino acids 1–181 and 1–190 of α and β chain respectively fused C terminally to the dimerizing nucleotide exchange factor GrpE (see additional file 1), this construct was not biotinylated and used in the LOCI competition assay. b: Determined using competitive LOCI assay. The reference column also states the MHC alleles known to bind the peptide.

Justesen et al. Immunome Research 2009 5:2   doi:10.1186/1745-7580-5-2