Immunome Research


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Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS

Maurizio Ceppi3,1,2,6, Giovanna Clavarino3,1,2, Evelina Gatti3,1,2, Enrico K Schmidt3,1,2, Aude de Gassart3,1,2, Derek Blankenship5, Gerald Ogola5, Jacques Banchereau4, Damien Chaussabel4 and Philippe Pierre3,1,2*

Author Affiliations

1 Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Case 906, 13288 Marseille cedex 9, France

2 INSERM, U631, 13288 Marseille, France

3 CNRS, UMR6102, 13288 Marseille, France

4 Baylor Institute for Immunology Research (BIIR), 3434 Live Oak, Dallas, TX 75204, USA

5 Baylor Institute for Health Care Research and Improvement, 8080 North Central Expressway, Dallas, TX 75206, USA

6 Genomic Vision, Paris Santé Cochin, 75014 Paris, France

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Immunome Research 2009, 5:5 doi:10.1186/1745-7580-5-5

Published: 27 November 2009

Additional files

Additional file 1:

Shows the validation of our microarray analysis in the human moDCs system.

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Open Data

Additional file 2:

(A-D) shows a detailed description of the genes subsets related to the global alterations of total and polysomal-bound mRNA in LPS-activated moDCs described in Fig. 3.

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Additional file 3:

Shows a heatmap of the preliminary list of 20'438 probe sets filtered on flags described in Fig. 4.

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Additional file 4:

Shows the complete list of the 375 probe sets with statistically significant interaction and is related to Fig. 4.

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Additional file 5:

(A-E) shows the validation of the array data by real time qPCR using Total and Polysomal RNA populations, and is related to Table 2.

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Additional file 6:

(A-D) shows correlations between Array and PCR Data after Spearman correlations.

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Additional file 7:

Shows the sequences of all employed primers for quantitative real-time PCR.

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Open Data