Figure 6.

Correlation between RPL26 mRNA translational disengagement and protein down-regulation in LPS-activated moDCs. (A) Gene expression of the RPL26 Total and Polysomal mRNAs determined by microarrays analysis (left) and confirmed by qRT-PCR analysis (right), depicted as fold induction between 4 h and 0 h and 16 h and 4 h post-LPS. (B) Immunoblot to assay RPL26 protein expression at 0 h, 4 h and 16 h post-LPS. An actin immunoblot is shown for equal loading control. The relative protein expression (top) has been determined by quantifying the immublot signals with the software ImageQuant (Fuji) and is representative of a typical experiment (n = 3).